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C.A.P.T.U.R.E. (CLARITY-based Activity and Projection Tracking upon Recombination)

--- a platform that combines rapid whole brain clearing,  simple volumetric imaging,  molecular genetics and computational analysis to study circuit structure and functions in intact mammalian brains.
 

Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences. [PDF] 

 

Ye L*, Allen WE*,Thompson KR*, Tian Q, Hsueh B, Ramakrishnan C, Wang A-C, Jennings JH, Adhikari A, Halpern CH, Witten IB, Barth AL, Luo L, McNab JA, and K Deisseroth.  Cell, Volume 165, Issue 7, p1776–1788, 16 June 2016.

 

 

 

 

CAPTURE for TRAP (activity-based cell body detection and analysis)

 Schematic of ArcTRAP labeling and the enhanced cohort-scale CLARITY pipeline for rapid whole brain clearing and imaging

Schematic of ArcTRAP labeling and the enhanced cohort-scale CLARITY pipeline for rapid whole brain clearing and imaging

 

CAPTURE for axonal projection analysis

 Schematic of viral labeling (top). Three-dimensional rendering of a CLARITY mouse hemisphere, visualizing outgoing mPFC projections (lower left) and example of CLARITY-based tractography (lower right).

Schematic of viral labeling (top). Three-dimensional rendering of a CLARITY mouse hemisphere, visualizing outgoing mPFC projections (lower left) and example of CLARITY-based tractography (lower right).